Yangyan Yin1,2,3†, Chunling Li1,2,3†, Zhe Pei4, Changting Li2,3, Zhongwei Chen2,3, Huili Bai1,2,3, Chunxia Ma1,2,3, Meiyi Lan1,2,3, Jun Li2.3, Yu Gong5, Jing Liu5, Ling Teng1,2,3, Leping Wang2,3, Zhongsheng Qin2,3, Ezhen Zhang6,*, Hao Peng2,3,*.
1Institute of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China
2Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, Guangxi, China
3Key Laboratory of China (Guangxi)-ASEAN Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, 530001, Guangxi, China
4Virginia Polytechnic Institute and State University, VA, 24061, USA
5Guizhou Provincial Livestock and Poultry Genetic Resources Management Station, Guiyang, 550001, Guizhou, China
6Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China
†These authors contributed equally to this work.
*These authors contributed equally as corresponding authors.
Abstract
Lactobacilli, recognized as beneficial bacteria within the human body, are celebrated for their multifaceted probiotic functions, including the regulation of intestinal flora, enhancement of body immunity, and promotion of nutrient absorption. This study comprehensively analyzed the genotypic and phenotypic characteristics of Lactiplantibacillus plantarum (L.plantarum) strains isolated from the intestines of healthy chicks, and assessed their potential as probiotics. The assembled genome consists of 2,952,1986 bp, and a total of 1,771 coding sequences (CDSs) were predicted. Based on the entire genome sequence analysis, 50 stress resistance genes and 7 virulence factors were identified. The results of the phenotypic experiments showed that the strain had good resistance to high temperature, low temperature, acid, alkali, salt, artificial gastrointestinal fluid, and strong antioxidant capacity. Additionally, transcriptomic analysis confirmed that under stress conditions, the expression levels of key genes were significantly upregulated. Therefore, the phenotypic characteristics of L.plantarum GX17 align well with its genotypic features, demonstrating promising probiotic properties. This strain holds great potential as a probiotic candidate, and further investigation into its beneficial effects on human health is warranted.
Keywords Lactiplantibacillus plantarum GX17, Genome-wide, Gene prediction, Stress resistance genes, Stress resistance