Ying Zhou1,2†,Shuo Zhao1†,Longhua,Liang3†,Yibin Qin1,Ke Cheng3,Zhongwei Chen1,Bingxia Lu1,Qunpeng Duan1,Ying Peng1,Yu Huang4,Zhenhua Duan4,Kaiyi Jiang1,2,Xunye Yang1,Ying He1
1、Guangxi Key Laboratory of Veterinary Biotechnology, Key Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Guangxi Veterinary Research Institute, Ministry of Agriculture and Rural Affairs of China, Nanning, China;
2、College of Animal Science and Technology, Guangxi University, Nanning, China;
3、Guangxi National Farm Yongxin Animal Husbandry Group Co., Ltd., Nanning, China;
4、Yulin Animal Disease Prevention and Control Center, Yulin, China.
ABSTRACT： Mycoplasma hyopneumoniae (Mhp) infection significantly challenges Guangxi’s pig farms, yet its prevalence and molecular characteristics remain poorly understood. This study aimed to define circulating Mhp genotypes and their distribution in the region.From 2022–2023, 1,362 pig lung samples were randomly collected from 14 Guangxi regions. Mhp was detected using TaqMan Real-time PCR. Strong positive samples underwent multilocus sequence typing (MLST) of adk, rpoB, and tpiA genes to assess genetic relationships.Of 1,362 samples, 655 (48.1%) were Mhp-positive. MLST amplification succeeded for 61 samples, revealing 27 sequence types (24 novel) across all 14 regions. Phylogenetic analysis indicated predominant circulation of Mhp types I and V. High Mhp incidence and substantial genetic diversity were observed.This study provides comprehensive analysis of Mhp in Guangxi, revealing high prevalence and genetic diversity dominated by types I and V. These findings expand understanding of Mhp epidemiology in China and offer a theoretical foundation for developing prevention and control strategies in Guangxi.
Keywords：Mycoplasma hyopneumoniae；genotyping；MLST；swine epidemiology；Guangxi
Frontiers in Veterinary Science,2025, 12: 1619301.
doi：doi.org/10.3389/fvets.2025.1619301